In the early 1950s, experiments by two teams of researchers, Salvador Luria working with Mary Human and Joe Bertani working with Jean Weigle, showed that some strains of bacteria were more resistant ...

Scientists first observed the biological phenomenon of restriction and modification in the 1950s, but they did not identify and isolate restriction enzymes until the late 1960s. 3 In 1970, Hamilton ...

Cold Spring Harbor, NY-- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of Restriction Enzymes: A History, available on its website in hardcover format. Restriction enzymes cleave ...

Enzymes that cut DNA at specific sites are called endonucleases; these enzymes play many roles in genomic replication, fidelity, and defense. The initial discovery of restriction endonucleases in the ...

The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...

Most restriction endonucleases use Mg²⁺ to hydrolyze phosphodiester bonds at specific DNA sites. We show here that Bfil, a metal-independent restriction enzyme from the phospholipase D superfamily, ...

Bacteria and the viruses that infect them are engaged in a molecular arms race as ancient as life itself. Evolution has equipped bacteria with an arsenal of immune enzymes, including CRISPR-Cas ...

Grapevine dieback, caused by Lasiodiplodia theobromae, is an important trunk disease worldwide. Transformants of L. theobromae were generated in an attempt to identify potential pathogenicity-related ...